The expression of HER-2 protein,gene amplification and its clinical significance in malignant melanoma
Abstract：Objective To investigate the expression of HER-2 protein and gene amplification in malignant mel-anoma and evaluation of HER-2 in malignant melanoma occurrence,development and prognosis.Methods Using PV-9000 two steps method and in situ chemical hybridization to detect the expression of HER-2 protein,gene amplification in paraffin-embedded tissue chip material.Results The positive rate of HER-2 expression in malig-nant melanoma,pigmented nevus were 76.9%,25.0%;The positive rate of HER-2 gene amplification were 75. 0%,30.0%.There were significant differences between malignant melanoma and pigmented nevus in the expres-sion and amplification of HER-2 (χ2 =12.480,P=0);The expression and amplification of HER-2 in malignant melanoma had correlation with the depth of invasion and the lymph node metastasis (P<0.05).Conclusion HER-2 is closely related to the occurrence and development of malignant melanoma,and can be considered as an im-portant index for determing the biological behavior of malignant melanoma.
Keywords：Malignant melanomaHER-2ImmunohistochemistryFluorescence
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 704-707 )