Examination and identification of macroamylasemia

Bu Ling; Sun Yi; Sun Lingxian; Han Yiwen; Li Jiang

doi:10.3969/j.issn.1674-7151.2025.03.020

Abstract：Objective To explore a simple and rapid diagnostic and differential method for macroamylasemia and eliminate the interference of macroamylase in clinical test results.Methods The clinical data of three patients with suspected macroamylasemia who visited Jiangsu Province Official Hospital from July 2020 to January 2023(cases 1,2 and 3)were collected and included in experimental group.Two patients with confirmed acute pancreatitis(cases 4 and 5)were included in control group.Before and after precipitation of giant amylase by modified polyethylene glycol(PEG)precipitation method,the level of serum amylase(AMY)was detected using dry chemical detection system and wet chemical detection system,respectively,and the detection results were compared.Results After PEG precipitation,the results of AMY in cases 1,2 and 3 of experimental group detected by wet chemical detection system decreased significantly compared with those before precipitation,with the decrease rates of 94%,97%and 97%,and the results of AMY in cases 4 and 5 in control group was reduced with a decrease rate of 18%and 13%.After PEG precipitation,the AMY levels of cases 1 and 2 in experimental group and cases 4 and 5 in control group detected by dry chemical detection system were decreased compared with those before precipitation,with the decrease rates of<10%,<10%,30%and 34%respectively.However,the level of AMY in case 3 of experimental group decreased after PEG precipitation,with a decrease rate of>86%.Before PEG precipitation,compared with the results of wet chemical detection system,the AMY levels of cases 1 and 2 in experimental group detected by dry chemical detection system decreased significantly,with the decrease rates of 92%and>95%,respectively.Before PEG precipitation,the results of AMY detected by dry chemical detection system for case 3 in experimental group and cases 4 and 5 in control group decreased by 3%,3%and-3%respectively compared with those detected by wet chemical detection system.After PEG precipitation,the AMY detection results of cases 1,2,4 and 5 by wet chemical detection system were reduced compared to those by dry chemical detection system before precipitation,with decrease rates of 44%,<10%,16%and 16%,respectively.However,the AMY level of case 3 in experimental group was significantly reduced by wet chemical detection system after PEG precipitation,with a decrease rate of 97%compared to that of dry chemical detection system.Conclusion The PEG precipitation method is a simple and effective way to identify amylasemia,and dry chemical detection system could be used to screen for macroamylase with relatively high molecular weight.