Results analysis on joint detection of six kinds of common respiratory viruses

Yang Jianfen

doi:10.3969/j.issn.1674-7151.2025.03.003

Abstract：Objective To review and analyze the results of joint detection of six kinds of common respiratory viruses in a hospital,discuss the prevalence and distribution of respiratory infections,and provide diagnostic and treatment basis for clinical practice.Methods The 11 531 samples of throat swab for joint detection of six kinds of common respiratory viruses in Lijiang People's Hospital from November 2023 to March 2025 were collected,and PCR-fluorescent probe method was used to qualitatively detect six kinds of viruses,such as influenza A virus(INFA),influenza B virus(INFB),respiratory syncytial virus(RSV),adenovirus(ADV),human rhinovirus(HRV)and Mycoplasma pneumoniae(MP).The detection rates of different viruses,as well as the detection rates in different quarters and age groups were compared.Results Out of samples of 11 531 respiratory and pharyngeal swab submitted for testing,4 685 samples were positive,with a positive rate of 42.74%.Among them,3 805 cases of single virus infection were detected,accounting for 81.22%of positive samples;781 cases were co-infected with two kinds of viruses,accounting for 16.67%of positive samples;97 samples were infected with a mixture of three kinds of viruses,accounting for 2.07%of positive samples;two samples were infected with a mixture of four kinds of viruses,accounting for 0.04%of positive samples.There were 1 233 cases of INFA,518 cases of IFNB,1 390 cases of RSV,830 cases of ADV,1 628 cases of HRV and 1 275 cases of MP detected in 11 531 samples sent for testing.RSV,HRV and MP had the highest detection rates in the fourth quarter,which were 20.59%,20.66%and 18.44%,respectively.The detection rates of INFA and INFB were the highest in the first quarter,which were 9.99%and 18.08%,respectively.RSV and HRV had the highest detection rates in infants and young children under 1 year old,which were 22.45%and 19.33%,respectively.The detection rate of INFA was the highest among elderly people over 60 years old,which was 21.31%.MP had the highest detection rate among adolescents aged 7-18 years old,which was 21.08%.Conclusion Pathogen testing should be conducted as early as possible for patients with respiratory infections,which could provide effective reference for clinical diagnosis and treatment of respiratory diseases and is worth further promotion and application.