Application of combined detection of blood routine indicators and C-reactive protein in diagnosis of acute appendicitis in children

Meng Xueqin; Wang Hui

doi:10.3969/j.issn.1674-7151.2025.02.016

Abstract：Objective To explore the application of combined detection of blood routine indicators and C-reactive protein(CRP)in the diagnosis of acute appendicitis in children.Methods The 98 children with acute appendicitis who received treatment at Liaocheng Second People's Hospital from June 2022 to June 2024 were included in research group.According to different diseases they suffered from,the children were divided into acute simple appendicitis group(44 cases),acute suppurative appendicitis group(28 cases)and acute gangrenous appendicitis group(26 cases).Additionally,98 healthy children who underwent physical examinations in the hospital during the same period were selected as control group.The venous blood samples were collected from two groups of subjects and fully automated blood cell analyzer was used to detect blood routine indicators[white blood cell count(WBC)and neutrophil ratio(NEU%)].The level of CRP was detected using specific protein analyzer and immune scattering turbidity.The levels of WBC,NEU%and CRP were compared between the research group and control group,as well as among children with different disease types of acute appendicitis.The children with acute appendicitis were as positive control and healthy children undergoing physical examinations were as negative control,the diagnostic results of WBC,NEU%,CRP alone and in combination for acute appendicitis were calculated and compared.Results The levels of WBC,NEU%and CRP in research group were significantly higher than those in control group[WBC(×109/L):11.04±5.63 vs.5.16±1.11;NEU%:(68.69±17.35)%vs.(46.79±10.24)%;CRP(mg/L):5.81(0.80,24.42)vs.1.00(0.50,1.00);all P<0.05].There were statistically significant differences in WBC,NEU%and CRP levels among children with different types of acute appendicitis.The WBC,NEU%and CRP levels in acute gangrenous appendicitis group and acute suppurative appendicitis group were significantly higher than those in acute simple appendicitis group[WBC(×109/L):12.23±5.47,14.82±6.20 vs.7.93±3.13;NEU%:(77.63±10.58)%,(81.42±12.58)%vs.(55.31±13.33)%;CRP(mg/L):38.21(16.33,68.31),8.04(1.68,19.82)vs.1.00(0.50,2.45);all P<0.05].The sensitivity and accuracy of combined detection of WBC,NEU%and CRP in diagnosing acute appendicitis were significantly higher than those of each indicator detected separately(sensitivity:63.3%vs.41.8%,51.0%,39.8%;accuracy:79.1%vs.68.9%,74.5%,67.9%;all P<0.05).There was no statistically significant difference in diagnostic specificity between combined and individual testing(94.9%vs.95.9%,98.0%,95.9%,all P>0.05).Conclusions Blood routine indicators(WBC and NEU%)and CRP,as inflammatory factors,are commonly abnormally elevated in children with acute appendicitis.The combination of blood routine testing and CRP detection has good diagnostic value for acute appendicitis in children and could assist in distinguishing the types of acute appendicitis.