Application effect of enzyme linked immunosorbent assay in screening antibodies against human immunodeficiency virus
Lin Yunxia
Abstract：Objective To investigate the effectiveness of enzyme linked immunosorbent assay(ELISA)in the human immunodeficiency virus(HIV)antibody detection.Methods A total of 150 suspected acquired immune deficiency syndrome(AIDS)patients who went to Center for Disease Control and Prevention of Shanxian County from January 2021 to December 2022 were selected as research objects.The blood samples of all patients were collected for HIV antibody testing,using colloidal gold method and ELISA method.The true positive cases and diagnostic efficacy of the two methods were compared.Results The 110 cases of AIDS patients were diagnosed by Western blotting.The true positive cases detected by colloidal gold method and ELISA method were 81 cases and 102 cases,respectively.The sensitivity,accuracy and negative predictive value of ELISA method for HIV antibody detection were significantly higher than those by colloidal gold method(sensitivity:92.73%vs.73.64%;accuracy:92.67%vs.76.00%;negative predictive value:82.22%vs.53.23%;all P<0.05).There were no statistically significant differences in the specificity and positive predictive value of HIV antibody detection between ELISA and colloidal gold methods(specificity:92.50%vs.82.50%;positive predictive value:97.14%vs.92.05%;both P>0.05).Conclusion ELISA could better assist in the diagnosis of AIDS by screening HIV antibodies.
Keywords：Acquired immune deficiency syndromeEnzyme linked immunosorbent assayHuman immunodeficiency virus antibody
Publication Date：2025-03-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 66-69 )