Correlation between aldehyde dehydrogenase-2 gene polymorphism and homocysteine with prognosis of acute ischemic cerebral infarction

Du Xin; Zheng Yuting; Li Hongliu; Ren Zhanjun; Li Yadong; Cai Qibo

doi:10.3969/j.issn.1674-7151.2025.01.009

Abstract：Objective To analyze the correlation between polymorphism of acetaldehyde dehydrogenase-2 gene and homocysteine(HCY)with prognosis of patients with acute ischemic cerebral infarction.Methods A total of 50 patients with acute ischemic cerebral infarction admitted to the Department of Neurology,the Third Affiliated Hospital of Qiqihar Medical College from January to October 2024 were selected as the research objects and included in the study group.Imaging examination was conducted on all patients,and the patients were divided into large infarction group(22 cases),small infarction group(16 cases)and lacunar infarction group(12 cases)according to different lesion sizes.In addition,50 patients without cerebrovascular disease were included in control group.The polymorphism of acetaldehyde dehydrogenase-2 gene was qualitatively detected using PCR-fluorescence probe method.The level of HCY in each group was detected using cyclic enzyme method.The patients were followed up for half a year and divided into good prognosis group(37 cases)and poor prognosis group(13 cases)according to the National Institutes of Health Stroke Scale(NIHSS)score.The gene distribution and HCY test results of each group were compared.Results The frequency of AA genotype at rs671 locus of acetaldehyde dehydrogenase-2 gene in research group was higher than that in control group,and the difference was statistically significant[18.0%(9/50)vs.2.0%(1/50),P<0.05].The frequency of AA genotype at rs671 locus of acetaldehyde dehydrogenase-2 gene in large infarction group was higher than those in lacunar infarction group and small infarction group,and the differences were statistically significant[31.82%(7/22)vs.8.33%(1/12),6.25%(1/16),both P<0.05].The level of HCY in research group was higher than that in control group,and the difference was statistically significant(μmol/L:11.41±1.17 vs.7.08±1.05,P<0.05).The frequency of AA genotype at rs671 locus of acetaldehyde dehydrogenase-2 gene in poor prognosis group was higher than that in good prognosis group,and the difference was statistically significant[46.15%(6/13)vs.8.11%(3/37),P<0.05].The level of HCY in poor prognosis group was higher than that in control group,and the difference was statistically significant(μmol/L:14.21±1.33 vs.9.14±1.12,P<0.05).Conclusion The polymorphism of acetaldehyde dehydrogenase-2 gene and the level of HCY in patients with acute ischemic cerebral infarction are helpful to accurately evaluate the condition and prognosis.