Value analysis of multiple interleukin indicators detection in early diagnosis of diabetic nephropathy

Wang Ruixue; Zhang Feng; Zhang Lin

doi:10.3969/j.issn.1674-7151.2025.01.007

Abstract：Objective To study the application value of combined detection of multiple interleukin(IL)indicators in the early diagnosis of diabetic nephropathy.Methods A retrospective study method was used,the clinical data of 100 patients with diabetes mellitus admitted in Jinan Integrated Traditional Chinese and Western Medicine Hospital from January to December 2023 were collected and the patients were divided into simple diabetes group and diabetic nephropathy group(50 cases in each group)according to whether they had kidney disease.Chemiluminescence immunoassay was used to determine IL-6,IL-8 and IL-10,enzyme linked immunosorbent assay(ELISA)was used to detect IL-17A,IL-23 and IL-35,and the levels of above indicators between two groups were compared.The sensitivity,accuracy,negative predictive value,positive predictive value and specificity were calculated,and the diagnostic efficacies of multiple IL indicators for diabetic nephropathy were analyzed.Results The levels of IL-6,IL-8,IL-10,IL-17A and IL-23 in diabetic nephropathy group were higher than those in simple diabetes group,and the level of IL-35 was lower than that in simple diabetes group[IL-6(ng/L):3.47±0.63 vs.2.20±0.40;IL-8(ng/L):60.18±5.43 vs.49.42±4.37;IL-10(ng/L):5.26±0.90 vs.4.28±0.74;IL-17A(ng/L):370.94±69.75 vs.311.09±43.46;IL-23(mg/L):47.64±4.15 vs.21.45±2.83;IL-35(ng/L):123.65±17.26 vs.149.94±28.70;all P<0.05].The sensitivity,accuracy and negative predictive value of combined detection of six IL indicators were 96.00%,97.00%and 96.08%,which were higher than those of each IL indicator alone.The specificity of combined detection was 98.00%,which was higher than that of IL-10.The positive predictive value was 97.96%,which was higher than those of IL-8,IL-10,IL-17A and IL-23 alone,with statistically significant differences.Conclusion Through the joint test of IL-6,IL-8,IL-10,IL-17A,IL-23 and IL-35,a high diagnostic accuracy could be obtained,which has certain reference value for early clinical diagnosis of diabetes nephropathy.