Diagnostic value of nucleic acid detection for respiratory pathogenic bacteria in lower respiratory tract infection
Li Qunjuan
Bao Yan
Zhao Jihua
Gao Jing
Li Guohua
Abstract：Objective To analyze the diagnostic value of nucleic acid detection for respiratory pathogens in lower respiratory tract infection.Methods The clinical data of 500 children with suspected lower respiratory tract infection admitted in Qujing Maternal and Child Health Hospital from February 2023 to April 2024 were collected.Respiratory pathogens were detected using multiplex fluorescence PCR and microbial isolation and culture methods.The positive detectable rates were compared between the two methods,and consistency between the two methods was analyzed using Kappa test(κ>0.4 indicated consistency).Results The multiplex fluorescence PCR method detected 321 positive cases,with a total positive rate of 64.20%(321/500),while microbial isolation and culture method identified 179 positive cases,with a total positive rate of 35.80%(179/500).The difference between the two methods was statistically significant(P<0.05).Consistency analysis for Klebsiella pneumoniae,Haemophilus influenzae and Pseudomonas aeruginosa detection showed κ values of 0.750,0.782 and 0.753,respectively,indicating high consistency(all P<0.05).Conclusion Nucleic acid detection for respiratory pathogens demonstrates a higher detectable rate in diagnosing lower respiratory tract infection,with simple operational procedures,providing a reliable basis for clinical diagnosis.
Keywords：Respiratory pathogenLower respiratory tract infectionMultiplex fluorescence PCR methodDiagnostic value
Publication Date：2025-03-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 27-30 )