Application value of blood group identification and hemolytic three items detection during pregnancy in early diagnosis of hemolytic disease of newborn

Yue Yinping; Hu Shuyao; Deng Luohua; Han Wei

doi:10.3969/j.issn.1674-7151.2024.02.016

Abstract：Objective To explore the application value of blood group identification and hemolytic three items detection in the early diagnosis of hemolytic disease of newborn(HDN).Methods The 2 136 pregnant women undergoing prenatal examination and delivery at Xuzhou Mining Group General Hospital from January 2022 to December 2023 were selected as research subjects,and their ABO and Rh(D)blood groups were identified.ABO blood group,Rh(D)blood group and hemolytic three items detection(direct antiglobulin test,serum free antibody test,antibody release test)were performed on newborns with jaundice symptoms.Results Among the 659 suspected HDN newborns,135 cases were finally diagnosed,with a detectable rate of 20.5%.Among them,55 cases(40.7%)were male and 80 cases(59.3%)were female,with a significant difference(P<0.05).Among the 135 newborns with HDN,126 cases(93.3%)were caused by ABO blood group system,8 cases(5.9%)by Rh blood group system(anti-D,anti-E,anti-Ec,anti-c),and 1 case(0.7%)by MNS system(anti-M).The proportion of female with ABO-HDN was higher than that of male(61.9%vs.38.1%),with a significant difference(P<0.05).The positive detectable rate of both serum free antibody test and antibody release test was the highest at 54.8%.Conclusions Blood group identification and hemolytic three items detection during pregnancy are beneficial for early detection of the risk of HDN.For mothers of type O,it is important to identify the blood group of fetus as early as possible.For pregnant women with blood type O,the blood type of the fetus and newborn should be determined as soon as possible.If the blood type is incompatibility,close attention should be paid and timely intervention treatment should be given.