Comparative analysis of stool examination results between automatic stool analyzer and manual review methods

Wu Lichun; Li Yong

doi:10.3969/j.issn.1674-7151.2024.01.018

Abstract：Objective To analyze the effect of automatic stool analyzer and manual review method in stool examination.Methods A total of 9402 fresh stool specimens from patients in the Second Hospital of Sanming City from September 2022 to August 2023 were collected and tested for fecal tangible components(white blood cells,red blood cells,fat globules and fungi)and occult blood using FA160 automatic stool analyzer and manual review method,respectively.The results of various parameters in stool examination were recorded for comparative analysis.Results The positive rates of white blood cells,red blood cells,fat globules,fungi and occult blood using automatic stool analyzer were 4.90%,2.67%,6.31%,4.54%and 25.34%,respectively.Comparatively,the positive rates of manual review method were 4.17%,2.22%,5.55%,5.29%and 25.23%,respectively.Except for fungi,which were detected by automatic stool analyzer with a lower positive rate of tangible components than that using manual review method,the positive rates of other parameters were higher,with statically significant differences(all P＜0.05).There was no significant difference in the positive rate of occult blood test(P＞0.05).The positive coincidence rates of positive specimen results were 91.07%for white blood cells,96.17%for red blood cells,94.83%for fat globules,78.07%for fungi,and 98.19%for occult blood,respectively.The negative coincidence rates were 98.85%,99.46%,98.90%,99.56%and 99.25%,respectively.The positive predictive values were 77.44%,80.08%,83.47%,90.87%and 97.77%,respectively.The negative predictive values were 99.61%,99.91%,99.69%,98.79%and 99.39%.Conclusions Although the results of stool samples detected by automatic stool analyzer have a high overall coincidence rate with the manual review method,the automated analyzer still exhibits instances of both missed detection and false detection.Therefore,it is necessary to check the results of automatic analysis manually to improve the accuracy of the test results,and to provide reliable references for clinical practice.