Observation on results of different types of blood samples in biochemical test
Xu Congyu
Abstract：Objective To investigate the results of biochemical test in different blood samples.Methods The 117 healthy examinees in Pinghu Danghu Street Community Health Service Center from October to December 2022 were selected as research objects,and serum samples were collected.The differences in results of alanine transaminase(ALT),aspartate aminotransferase(AST),fasting blood glucose(FBG),lactate dehydrogenase(LDH)and creatine kinase(CK)obtained at different delivery time(within 15 minutes or after 60 minutes of blood collection)were compared.The results of LDH,CK,AST,uric acid(UA),serum creatinine(SCr)and total bilirubin(TBil)in normal and hemolytic samples were analyzed.Different anticoagulation treatments(untreated,heparin lithium anticoagulation and sodium citrate anticoagulation)were performed on serum samples,and the differences in results of ALT,AST,alkaline phosphatase(ALP),total protein(TP),albumin(ALB),triglycerides(TG),total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C)and low-density lipoprotein cholesterol(LDL-C)among different groups were compared.Results The ALT,AST,FBG,CK and LDH results obtained after 60 minutes of blood collection were slower than those from qualified samples[ALT(U/L):16.59±2.23 vs.19.65±2.22;AST(U/L):16.74±2.80 vs.20.71±2.85;FBG(mmol/L):4.92±2.64 vs.5.50±2.63;CK(U/L):70.05±2.80 vs.95.66±2.34;LDH(U/L):163.81±2.30 vs.205.32±2.21;all P＜0.05].The results of LDH,CK,AST and TBil in normal samples were lower than those in hemolytic samples,while the results of UA and SCr were higher than those in hemolytic samples[LDH(mmol/L):169.40±3.12 vs.297.20±3.26;CK(mmol/L):130.80±2.98 vs.265.40±3.02;AST(U/L):20.00±2.25 vs.102.10±2.30;TBil(mmol/L):12.22±2.33 vs.19.02±2.36;UA(mmol/L):300.40±2.23 vs.205.63±2.15;SCr(mmol/L):63.00±2.50 vs.34.58±2.23;all P＜0.05].The ALT,AST,ALP,ALB,TG,TC and HDL-C results obtained from samples treated with lithium heparin and sodium citrate anticoagulation showed statistically significant differences compared to serum samples without anticoagulation treatment.Conclusions There are many factors that affect the results of biochemical tests.In order to further improve the accuracy of biochemical test results,it is necessary to explore the impact of relevant factors in blood tests on the results,strengthen professional training,and improve the efficiency of blood sample test.
Keywords：Biochemical testBlood samplePrecision
Publication Date：2024-03-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 61-64 )