Interfering effect of heterophil antibodies on detection of thyroid function indexes
Zhang Feng
Abstract：Objective To analyze the interfering effect of heterophilic antibody(HAb)on detection of thyroid function indexes.Methods A total of 280 patients with inappropriate increased level of plasma thyroid stimulating hormone(TSH)in Jinan Hospital of Integrated Chinese and Western Medicine from July 2020 to July 2022 were selected as research objects.Among them,20 patients were suspected with HAb hyperplasia.TSH was detected using radioimmunoassay(RIA)and immunoradiography(IRMA).After excluding interference from serum rheumatoid factors,the level of HAb in serum samples was measured.The levels of free triiodothyronine(FT3)and free tetraiodothyronine(FT4)were detected using solid-phase radioimmunoassay.Results Using RIA and IRMA methods,the TSH level in patients with suspected HAb hyperintensities was lower than that in patients with improper TSH increase[mU/L:RIA 5.52±0.65 vs.16.08±3.10;IRMA 4.02±0.42 vs.16.03±2.96;both P＜0.05].There was no significant difference in FT3 and FT4 levels between patients with suspected HAb hyperplasia and other patients with improper TSH increase[FT3(ng/L):3.25±0.85 vs.3.21±0.66;FT4(ng/L):1.38±0.22 vs.1.38±0.22;both P＞0.05].Conclusion HAb might affect the detection results of thyroid function indicators,and analysis should be conducted based on actual conditions to reduce or exclude the impact.
Keywords：Thyroid functionInterfering effectHeterotropic antibody
Publication Date：2024-03-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 58-60 )