Application of peripheral blood atypical lymphocyte ratio combined with EB virus DNA load in diagnosis of infectious mononucleosis in children
Shen Xiaoming
Abstract：Objective To study the application of peripheral blood atypical lymphocyte ratio(ALY)combined with EB virus(EBV)DNA load in the diagnosis of infectious mononucleosis(IM)in children.Methods A total of 62 children with IM and 62 healthy children undergoing physical examination in Longyan People's Hospital from March 2020 to March 2022 were enrolled in IM group and healthy control group,respectively.The levels of peripheral blood ALY,lymphocytes count(LYM)and lymphocyte ratio(LYM%)in two groups of children were detected by microscope,and the level of EBV-DNA load was detected by real-time fluorescence quantitative polymerase chain reaction(PCR).The correlation between LYM,LYM%,peripheral blood ALY and EBV-DNA load was analyzed by Pearson correlation coefficient.The receiver operator characteristic(ROC)curve was drawn,the area under ROC curve(AUC)was calculated,and the diagnostic value of peripheral blood ALY combined with EBV-DNA load for IM was analyzed.Results The levels of peripheral blood ALY,LYM,LYM%and EBV-DNA load in IM group were significantly higher than those in healthy control group[peripheral blood ALY:(25.62±8.31)%vs.(18.01±4.62)%;LYM(×107/mL):8.35±2.61 vs.5.54±0.34;LYM%:(50.35±10.36)%vs.(37.69±6.31)%;EBV-DNA load(copies/mL):7.85±2.64 vs.5.31±1.10;all P＜0.05].The results of Pearson correlation analysis showed that the levels of LYM,LYM%and peripheral blood ALY were significantly positively correlated with EBV-DNA load in IM group(r values were 0.424,0.490 and 0.632,P values were 0.016,0.010 and 0.008).The AUC of peripheral blood ALY for diagnosis of IM was 0.773,95%confidence interval(95%CI)was 0.680-0.866,the sensitivity was 48.00%,and the specificity was 72.00%.The AUC of EBV-DNA load for diagnosis of IM was 0.810,95%CI was 0.718-0.902,the sensitivity was 60.00%,and the specificity was 96.00%,respectively.The AUC of combined detection of peripheral blood ALY and EBV-DNA load was the highest,which was 0.904,95%CI was 0.843-0.964,the sensitivity was 90.00%,and the specificity was 92.00%,respectively.Conclusion The peripheral blood ALY and EBV-DNA load are significantly increased in children with IM,which could be applied in the auxiliary diagnosis of IM.
Keywords：Peripheral blood atypical lymphocyte ratioInfectious mononucleosis in childEB virus DNA load
Publication Date：2024-03-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 11-14 )