Correlations of serum hepatitis B virus-DNA detection with liver function enzymological indicators in patients with hepatitis B
Liu Shaofang
Abstract：Objective To discuss the correlation of serum hepatitis B virus(HBV)-DNA with liver function enzymological indicators in patients with hepatitis B.Methods The 85 patients with hepatitis B admitted to Jiexi County Hospital of Traditional Chinese Medicine from April 2022 to May 2023 were selected as observation group,and over the same time period,85 healthy checkups were included as control group.Using fluorescence polymerase chain reaction(PCR),the serum HBV-DNA load was detected.Enzyme linked immunosorbent assay(ELISA)was used to detect alanine aminotransferase(ALT),α-L-fucosidase(AFU),aspartate aminotransferase(AST),prothrombin time(PT),hepatitis B surface antigen(HBsAg),hepatitis B e antibody(HBeAb),hepatitis B surface antibody(HBsAb),hepatitis B e antigen(HBeAg)and hepatitis B core antibody(HBcAb).According to the different viral loads,hepatitis B patients were assigned to negative group(HBV-DNA<5.0×102 U/mL;45 cases),low copy group(HBV-DNA was 5.0×102 to<1.0×104 U/mL;17 cases),mediate copy group(HBV-DNA was 1.0×104 to<1.0×106 U/mL;13 cases)and high copy group(HBV-DNA was 1.0×106 to 1.0×108 U/mL;10 cases).The quantitative detection results and positive detectable rates of HBV-DNA under different serological modes were compared,as well as the positive rates of HBV-DNA and HBeAg.The differences in liver function indicators among different groups were compared.Results Among 56 patients with HBsAg,HBeAb and HBcAb positive,19 cases with positive HBV-DNA were detected[positive rate of 33.93%,HBV-DNA of(4.27±0.13)×106 U/mL].Among 15 patients with HBsAg,HBeAg and HBcAg positive,11 cases with positive HBV-DNA were detected[positive rate of 73.33%,HBV-DNA of(2.34±0.11)×108 U/mL].Among 10 patients with HBsAg,HBeAg,HBeAb and HBcAb positive,7 cases with positive HBV-DNA were detected[positive rate of 70.00%,HBV-DNA of(1.55±0.09)×107 U/mL].Among 4 patients with HBsAg and HBcAb positive,one case with positive HBV-DNA was detected[positive rate of 25.00%,HBV-DNA of(9.41±0.52)×105 U/mL].Among 85 patients with hepatitis B,the positive rate of HBV-DNA was significantly higher than that of HBeAg(44.71%vs.29.41%,P<0.05).The levels of ALT,AFU,AST and PT in negative group,low copy group,mediate copy group and high copy group of patients with hepatitis B were significantly higher than those in control group[ALT(U/L):29.48±5.14,34.16±5.22,35.21±4.69,35.79±5.61 vs.19.52±3.02;AFU(U/L):33.27±4.16,40.66±7.03,66.54±6.57,92.57±12.49 vs.21.48±3.27;AST(U/L):35.29±5.44,50.37±6.29,75.39±8.36,90.25±10.32 vs.19.54±5.03;PT(s):12.46±1.36,13.29±1.02,16.54±1.88,20.43±2.19 vs.11.24±0.16;all P<0.05].Wtih the higher viral loads,AFU,ALT,AST and PT were gradually increased(all P<0.05).Conclusion Clinically,it is necessary to regularly detect the level of HBV-DNA in patients with hepatitis B,and timely understand the viral replication and changes in liver function indicators.
Keywords：Hepatitis BHepatitis B virus-DNALiver function indicator
Publication Date：2023-12-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 418-421 )