Analysis on Mycoplasma culture and drug sensitivity test results in cervical secretions of women of reproductive age

Deng Lian; Lin Lu; Zhang Chunchun

doi:10.3969/j.issn.1674-7151.2023.04.016

Abstract：Objective To collect cervical secretions from women of childbearing age,conduct mycoplasma culture and drug sensitivity tests,and analyze the results,providing a basis for the rational use of antibiotics.Methods The 2 000 women of childbearing age who underwent physical examinations at the gynecology outpatient department of Longyan People's Hospital from January 2022 to January 2023 were selected as study subjects.Cervical secretions from all study subjects were collected,and Ureaplasma urealyticum(UU)and Mycoplasma hominis(MH)were isolated,identified and counted,and drug sensitivity test kits were used to carry out Mycoplasma culture identification and drug sensitivity tests.The distribution of Mycoplasma in different age groups was analyzed.Results After cultivation,it was found that a total of 783 Mycoplasma positive samples(39.15%)were detected in 2 000 specimens,of which 745 samples were positive for UU,10 samples were positive for MH,and 28 samples were positive for UU+MH.In the distribution of Mycoplasma in different age groups,the detectable rates of Mycoplasma in 20-29 and 30-39 years old women were 41.68%and 42.10%,respectively.The detectable rates in 40-49 and<20 years old women were 23.51%and 19.44%,respectively.The results of the drug sensitivity test showed that UU was sensitive to Methicillin,Doxycycline,Tetracycline,Clarithromycin and Doxorubicin,while resistant to Azithromycin,Ciprofloxacin,Roxithromycin,Levofloxacin,Sparfloxacin,Gatifloxacin and Moxifloxacin.When a mixed infection of UU+MH occured,there was a high sensitivity to Tetracycline,Doxycycline and Minocycline,while resistance to other antibiotics was higher.Conclusions Through the cultivation of Mycoplasma in cervical secretions of women of childbearing age,the results showed that the detectable rate of UU is the highest,and the proportion of Mycoplasma detection was highest in 20-39 years old women.In drug sensitivity tests,positive individuals are more sensitive to Doxycycline,Tetracycline and Minocycline,so they could be used as preferred medication for treating Mycoplasma infection.