Related application on pollution treatment of amplification products in novel coronavirus nucleic acid detection by real-time polymerase chain reaction

Yan Xiaofeng; Li Qingwei; Sun Yuqiang; Rong Weina

doi:10.3969/j.issn.1674-7151.2023.04.009

Abstract：Objective To explore the related application of fluorescent polymerase chain reaction(PCR)in the treatment of amplification product pollution in detection of novel coronavirus(SARS-CoV-2)nucleic acid.Methods The models of SARS-CoV-2 gene detection amplification products pollution,physical surface pollution and space(aerosol)pollution were established to compare the removal effects of different chemical reagents and physical ultraviolet(UV)radiation treatments on physical surface pollution and space pollution.Results The circulation threshold(CT)value of 75%ethanol disinfectant group,nucleic acid scavenger group and different concentration of effective chlorine preparation groups were higher than that in control group,and the CT values of nucleic acid scavenger group were the highest,with CT values for glass,plastic and metal materials of 34.74±2.63,34.68±3.25 and 35.71±3.07,respectively.The CT values of different materials in 500,1 000,1 500,2 000 and 2 500 mg/L effective chlorine formulation groups were higher than those in 75%ethanol disinfectant group,and the higher the concentration of effective chlorine,the greater the CT values,with statistically significant differences.The CT values of each group exposed to long-term UV radiation at different time were higher than those of control group,and among them,the CT values of 120 minute UV irradiation group were the highest,with the CT values for glass,plastic and metal materials of 35.51±2.70,35.49±2.63 and 35.55±2.58,respectively.The longer the UV irradiation time,the larger the CT values,with statistically significant differences.The CT value of the ventilation + UV irradiation for 120 minutes + 75%ethanol disinfectant + nucleic acid scavenger + 2 500 mg/L effective chlorine preparation group after treatment was 36.98±3.47,which was significantly higher than that before treatment and other groups,and the differences were statistically significant.Conclusions There are many amplified products,but nucleic acid scavenger,2 500 mg/L effective chlorine and 120 minutes could be selected for UV irradiation.The treatment mode of space pollution environment could be selected ventilation + UV irradiation 120 minutes + 75%alcohol + nucleic acid scavenger + 2 500 mg/L effective chlorine combination mode,which is worthy of attention.