Serological study of clinical Rh phenotype and necessity research of homotransfusion in patients with repeated transfusion

Chen Zhen; Zhang Jirong

doi:10.3969/j.issn.1674-7151.2023.04.003

Abstract：Objective To analyze the distribution of Rh serum phenotype in Pingyi County of Linyi City,and evaluate the necessity of detection of five Rh antigens in accurate homologous transfusion,in order to provide more evidence for the safety of clinical blood transfusion.Methods The 4 710 blood donors and 5 417 blood users in Pingyi County,Linyi City from January 2020 to March 2023 were selected as research subjects.The ABO blood type classification and Rh blood type system antigen classification of blood donors and users were detected,and the occurrence of adverse reaction events,production of irregular antibodies,and relationship between Rh typing antigens and irregular antibodies were analyzed.Results ① The proportions of ABO type in blood donors were as follows:A type accounted for 30.53%,B type for 28.48%,O type for 32.24%,AB type for 8.75%;blood users:A type for 31.42%,B type for 25.63%,O type for 34.56%,AB type for 8.32%.② The Rh typing antigen CCDee phenotype was the most common type in blood donors,same as that in blood users.③ From 2020 to 2023,the proportion of adverse reactions after transfusion in blood users showed a decreasing trend year by year,while the irregular antibody content showed an increasing trend,and the anti-E antibody showed a decreasing trend.④ There were no correlations between the positive rate of Rh typing antigen and Rh antibody detection,Rh antibody detection and anti-E antibody detection,and Rh typing antigen positive rate and irregular antibody detection in blood users(r values were 0.875,0.900 and 0.700,and P values were 0.067,0.083 and 0.233,respectively).Conclusion Homologous transfusion of five Rh antigens could effectively improve transfusion efficacy,reduce the production of blood group specific antibodies,and reduce the incidence of adverse reactions,which is worthy of clinical promotion.