The role of miR-21 in human breast cancer MCF-7 cells invasion by regulating STAT3 expression
ZHAO Shou-xiang
WANG Tao
LI Yu-jun
Abstract：Objective To identify expressions of miR-21 and signal transducer and activator of tran-scription 3 (STAT3) in human breast cancer MCF-7 cells and explore the role of miR-21 on cell invasion which regulate expression of STAT3. Methods MCF-7 cells were cultured in vitro. Cell morphology was ob-served by phase contrast microscope and the expression of STAT3 in MCF-7 cells was determined by im-munofluorescence cytochemistry method. MiR-21 which regulated expression of STAT3 was predicted by bioinformatics and identified with luciferase assay. After transfection of miR-21 mimics into cells , the expres-sions of miR-21 and STAT3 were determined by qRT-PCR and Western blot. The invasion of MCF-7 cells was detected in vitro by transwell chamber. Results The MCF-7 cell grew flakily with prominency under light mi-croscope. Expression of STAT3 was positive by immunofluorescence cytochemistry. MiRanda and TargetScan showed that miR-21 was well complementary with STAT3 gene. MiR-21 mimics could inhibit STAT3 mRNA expression showed by luciferase assay. Results of qRT-PCR and Western blot showed that over-expression of miR-21 down-regulated expression of STAT3 mRNA and protein. The invasion of MCF-7 cells was suppressed after transfection of miR-21 mimics. Conclusion MiR-21 may negatively regulate STAT3 expression in hu-man breast cancer MCF-7 cells and inhibit cells invasion.
Keywords：miRNASignal transducer and activator of transcription 3Breast cancer MCF-7 cellsBioinformatics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )