Clinical application of real-time fluorescence quantitative PCR detection in Mycobacterium tuberculosis pathological diagnosis
LIN Ying
CHEN Xiao-yan
YU Xun-bi n
Abstract：Objective To study clinical application of real-time fluorescence quantitative PCR detec-tion in Mycobacterium tuberculosis (MTB) pathological diagnosis. Methods 144 patients with tuberculosis (TB) were collected in our hospital from May 2013 to February 2015. Paraffin-embedded human tissues of TB patients were detected by histopathological examination , acid-fast staining and FQ-PCR method. And positive rates of all methods were analyzed statistically. Results In 144 patients with the clinical diagnosis of TB, 68 cases (47.22%) were positive for MTB by FQ-PCR, 22 cases (15.28%) were positive for acid-fast staining and of which 74 cases (51.39%) for histopathological examination. The differences had statistical significance (P<0.01). The positive rates of MTB by FQ-PCR method in lung biopsy samples, lobectomy specimens and pleural resection specimens were all higher than those of histopathological examination and acid-fast staining. The positive rates of MTB by FQ-PCR method in lymph node biopsy and other tissue samples were higher than that of acid-fast staining, and lower than that of histopathological examination. Conclusion FQ-PCR assay can be effective in the classification of TB, and it is more sensitive than other special staining methods (acid-fast), which provides liable basis for the pathological diagnosis.
Keywords：Mycobacterium tuberculosisTuberculosisReal-time fluorescence quantitative PCRAcid-fast stainingHistopathology
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 143-147 )