Detection of gene qacEΔ1 in Acinetobacter baumannii and Pseudomonas aeruginosa and study on the resistance of disinfectant

doi:10.3969/j.issn.1674-7151.2015.03.003

Abstract：Objective To understand the status of Acinetobacter baumannii and Pseudomonas aerugi-nosa carrying resistance to disinfectants gene qacEΔ1 and the resistance to three kinds of commonly used dis-infectants. Methods 30 strains Acinetobacter baumannii and 30 strains Pseudomonas aeruginosa in our hos-pital from January 2012 to December 2014 were collected. The status of carrying resistance to disinfectants gene qacEΔ1 of two kinds bacteria were detected by PCR method, and the effect of qacEΔ1 gene to the resis-tance of three kinds commonly used disinfectants were examined by quantitative germicidal test. Results The positive rates of Acinetobacter baumannii and Pseudomonas aeruginosa carrying resistance to disinfectants gene qacEΔ1 were 73.3%(22/30) and 60.0%(18/30), respectively. There was no standard strains carrying gene qacEΔ1. The disinfection rates of active iodine, active chlorine and fast speed handdisinfectant to Acinetobac-ter baumannii and Pseudomonas aeruginosa were all showed dose-effect relationship and time-effect relation-ship. At 1 min, there were all no statistical significance in the differences of disinfection rate of active iodine to qacEΔ1 gene positive strains and negative strains of two kinds bacteria at each concentration (Pall>0.05) ex-cept 300 mg/L(Pall<0.05), and there were all had statistical significance in the differences of disinfection rate of active iodine to qacEΔ1 gene positive strains and negative strains of two kinds bacteria at others concentra-tion and action time (Pall<0.05). At 5 min and 7 min, the disinfection rates of active chlorine with each con-centration to Acinetobacter baumannii and Pseudomonas aeruginosa carrying qacEΔ1 gene were all lower than that of negative strains, and the differences all had statistical significance(Pall<0.05). At 1 min, the disinfec-tion rate of active chlorine at 500 mg/L to Acinetobacter baumannii carrying qacEΔ1 gene was lower than that of negative strains, and the difference had statistical significance (P<0.05), but there was no difference to Pseudomonas aeruginosa (P>0.05). There were all had statistical significance in the differences of disinfection rate of fast speed handdisinfectant to qacEΔ1 gene positive strains and negative strains of two kinds bacteria at each concentration and action time (Pall<0.05). Conclusion The isolation rate of Acinetobacter baumannii and Pseudomonas aeruginosa carrying resistance to disinfectants gene qacEΔ1 in our hospital are higher, and the resistance of qacEΔ1 gene positive strains to disinfectants are higher than that of negative strains and stan-dard strains.