The selection of serum protein markers for HBV-associated hepatocellular carcinoma

doi:10.3969/j.issn.1674-7151.2015.02.004

Abstract：Objective To select specific serum protein markers for the early diagnosis of hepatocellu-lar carcinoma(HCC). Methods 20 cases hepatitis B virus (HBV) associated HCC patients, 30 cases healthy patients, 20 cases chronic hepatitis B (CHB) patients, and 20 cases HBV associated hepatic cirrhosis from March 2013 to June 2014 in department of hepatobiliary surgery of our hospital were collected. The serum sam-ples of all the subjects were collected and digested by trypsin and then analyzed in homemade strong cation ex-change and reverse-phase two-dimensional capillary chromatographic column , and the results were analyzed statistically. Results 3578 kinds proteins were selected and 7 kinds HCC related serum markers including histidine-rich glycoprotein (HRG), hemoglobin subunit alpha, hemoglobin subunit beta, ceruloplasmi(CP), alpha-1B-glycoprotein(A1BG), antithrombin-Ⅲ(ATⅢ)and alpha-fetoprotein(AFP) were identified. Com-pared with HBV associated hepatic cirrhosis group, the expression levels of A1BG and AFP were higher and ATⅢwas lower in HBV associated HCC group, and the differences all had statistical significance(Pall<0.05). Compared with CHB group, the expression levels of CP and AFP were higher and hemoglobin subunit alpha was lower in HBV associated HCC group, and the differences all had statistical significance (Pall< 0.05). Compared with healthy control group, the expression level of AFP was higher and the HRG and hemoglobin subunit beta were lower, and the differences all had statistical significance (Pall< 0.05). Conclusion All these 7 kinds serum protein markers which synthesized in liver are all closely related with HCC incidence and pathogenesis. The proteomic fingerprint and detection of multi-markers provide new ideas for clinical liver tu-mor diagnosis.