The application of anti -gliadin antibody and anti -endomysial antibody immunoglobulin A, immunoglobulin G levels detection in diagnosis of gluten sensitive enterpathy

doi:10.3969/j.issn.1674-7151.2015.01.006

Abstract：Objective To evaluate the clinical value of anti-gliadin antibody IgG , IgA and anti-en-domysial antibody IgG, IgA in the diagnosis of gluten sensitive enterpathy (GSE). Methods 35 cases GSE patients (GSE group) and 50 cases healthy people (healthy control group) were selected from August 2012 to September 2014 in our hospital. The levels of anti-gliadin antibody IgG , IgA and anti-endomysial antibody IgG, IgA were measured by ELISA method and indirect immunofluorescence method, respectively. All data were analyzed statistically. Results The positive rates of anti-gliadin antibody IgG, IgA and anti-endomysial antibody IgG, IgA in GSE group were 25.7%(9/35), 25.7%(9/35), 14.3%(5/35) and 28.6%(10/35), re-spectively, which were all higher than that of healthy control group. And the differences all had statistical sig-nificance(Pall<0.05). The positive rates of anti-gliadin antibody IgG, IgA and anti-endomysial antibody IgG, IgA combined detection were 45.7%(anti-gliadin antibody IgG, IgA), 37.1%(anti-endomysial antibody IgG, IgA) and 48.6%(anti-gliadin antibody IgG, IgA+anti-endomysial antibody IgG, IgA), which were all higher than that of healthy control group. And the differences all had statistical significance (Pall<0.05). Anti-gliadin antibody IgG, IgA and anti-endomysial antibody IgG, IgA had high specificity and positive predictive value in the diagnosis of GSE, but had low sensitivity. The highest specificity and positive predictive value were all 100.0%(anti-endomysial antibody IgG, IgA). The sensitivity increased in combined detection of anti-gliadin antibody IgG, IgA and anti-endomysial antibody IgG, IgA, the highest was 48.6%(anti-gliadin antibody IgG, IgA+anti-endomysial antibody IgG, IgA). Conclusion Anti-gliadin antibody IgG, IgA and anti-endomysial antibody IgG, IgA combined detection can improve the sensitivity in the diagnosis of GSE, and reduce misdi-agnosis. The GSE high risk group should detect anti-gliadin antibody IgG , IgA and anti-endomysial antibody IgG, IgA to screening and diagnosis.