The correlation research between the expression of NLRP3-mRNA in patients with type 2 diabetes mellitus and carotid artery atherosclerosis
Abstract：Objective To study the correlation between the expression of NLR family, pyrin domain containing 3(NLRP3) mRNA in peripheral blood mononuclear cells (PBMCs) of patients with type 2 diabetes mellitus(T2DM) and carotid artery atherosclerosis (CAS). Methods 107 cases patients with T2DM, 35 cas-es patients with CAS and 35 cases healthy controls from June 2013 to December 2013 in our hospital were col-lected. The T2DM patients were divided into T2DM group (26 cases), T2DM combine mild plaque group (32 cases), T2DM combine midrange plaque group(38 cases), and T2DM combine severe plaque group(11 cas-es). The expression levels of NLRP3-mRNA in PBMCs in all the groups were detected by real-time fluorescent quantitative polymerase chain reaction, and the results were analyzed statistically. Results There was statis-tical significance in the difference of NLRP3-mRNA expression level among control group, T2DM group, T2DM combine CAS group and CAS group (P<0.01). The NLRP3-mRNA expression levels in T2DM group, T2DM combine CAS group and CAS group were all higher than that of control group , and the differences all had statistical significance(Pall<0.05). The NLRP3-mRNA expression level in T2DM combine CAS group was higher than that of T2DM group and CAS group, and the differences all had statistical significance (Pall<0.05). There was statistical significance in the difference of NLRP3-mRNA expression level among all the T2DM combine CAS groups (P<0.01). The NLRP3-mRNA expression levels in T2DM combine severe plaque group and T2DM combine midrange plaque group were all lower than that of T2DM combine mild plaque , and the differences all had statistical significance(Pall<0.05). The correlation research results showed that the NL-RP3-mRNA expression level was negative correlated with plaque maturation degree (r= -0.70, P< 0.01). Conclusion The NLRP3-mRNA is correlated with the occurrence and development of T2DM combine CAS.
Keywords：NLRP3Peripheral blood mononuclear cellsCarotid artery atherosclerosisType 2 dia-betes mellitusReal-time fluorescent quantitative polymerase chain reaction
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 13-16 )