Error analysis of susceptibility test in Acinetobacter baumannii to amikacin
Abstract：Objective To evaluate the test errors of VITEK-2 for antimicrobial susceptibility testing of Acinetobacter baumannii clinical isolates to amikacin. Methods The susceptibility of 115 Acinetobacter baumannii isolates were collected in our hospital from January 2014 to February 2014. Susceptibility teating was determined by VITEK-2 instrument, agar double dilution method and Kirby-Bauer method. The results were compared by using agar double dilution method as a reference method , and the test errors of VITEK 2 were analyzed. Results In 115 strains Acinetobacter baumannii, the main clinical departments were ICU (39.1%) and respiratory medicine(32.2%). The main specimen type was sputum specimen(84.3%). The main age was above age 50 (83.5%). Drug resistant rate of Acinetobacter baumannii to amikacin by agar double di-lution method was 62.6%(72/115), medium rate was 0.0%(0/115), sensitive rate was 37.4%(43/115). In comparison to the results of agar double dilution method, the susceptibility results of Kirby-Bauer method showed 100.0%consistent rate,while VITEK-2 method showed 62.6%consistent rate. VITEK-2 showed very major error (36.5%) and minor error (0.9%). Conclusion There is different degree error in susceptibility test of amikacin to Acinetobacter baumannii by VITEK-2 method, and agar double dilution method or Kirby-Bauer method is needed.
Keywords：Acinetobacter baumanniiSusceptibility testVITEK-2Agar double dilution methodKirby-Bauer method
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 176-178 )