The influence of RNAi on expression of GALNT7 to malignancy behaviours of cervical cancer cells

PENG Rui-qing; MEN Jian-long; REN Jing

doi:10.3969/j.issn.1674-7151.2014.03.005

Abstract：Objective To investigate the influence of polypeptide N-acetylgalactosaminyltransferase 7 (GALNT7) gene to growth, invasiveness and migration of cervical cancer cells. Methods GALNT7 gene was silenced by RNA interference, took non-specificity sequence pSilencer 2.1 as negative control. After transfection of cervical cancer cells (HeLa and C33A cells), Western Blot was used to detect protein expres-sion of GALNT7 gene, the growth ability was detected by MTT assay, the ability to form colonies was evaluated by the colony formation assay, the invasion and migration ability were detected by matrigel invasion assay. Re-sults In experimental group the protein expression of GALNT7 was decreased significantly than that of control group, and the differences all had statistical significance(Pall<0.05). MTT assay revealed that cell growth was inhibited, the inhibitory rates were decreased by19% in HeLa cells and 22% in C33A cells, and compared with control group, the differences all had statistical significance (Pall<0.05). The ability to form colonies in experimental group were inhibited by 56%in HeLa cells and 52%in C33A cells than that of control group , and the differences all had statistical significance(Pall<0.05). In experimental group, the inhibitory rates on inva-sion were decreased by 48%in HeLa cells and 54%in C33A cells , and the inhibitory rate on migration was decreased by 30%in HeLa cells, and compared with control group, the differences all had statistical signifi-cance (Pall<0.05). Conclusion RNAi mediated GALNT7 down-regulation can inhibit the growth, invasion and migration of cervical cancer, and can be an important target to cervical cancer gene-therapies.