The constrution and clinical application of convenient Candida albicans cultivation method

doi:10.3969/j.issn.1674-7151.2014.03.002

Abstract：Objective To study the clinical application value of a convenient fungus cultivation method. Methods The suspected samples of fungus infection from the clinic service and hospitalization pa-tients in our hospital from January 2006 to December 2013 were collected. The fungus infection samples were detected by convenient fungus cultivation method. The cultivated results of homemade culture media were com-pared with conventional pour-plate method. The detection rates of fresh homemade culture media and culture media saved 6 months at 2~8℃were compared. Results The Candida albicans detection rates of homemade culture media and conventional culture media were 38.4%and 26.1%, respectively among 307 samples from 2011 to 2013. The detection rates of homemade culture media in vaginal secretions and sputum samples were better than that of conventional pour-plate method, and the differences all had statistical significance (Pall<0.05). There was no statistical significance in the detection rates of urine and pharyngeal swab samples between two methods(Pall>0.05). The colonial morphology were plesiomorphism, and almost the other bacterium were depressed in the two culture medias. The colonial morphology shown blue in conventional culture media , but shown cream in homemade culture media. There were no statistical significance in sputum sample ,vaginal se-cretions and the other samples between fresh homemade culture media and culture media saved 6 months at 2~8℃(Pall>0.05). The detection rate of the other pathogenic bacterium except Candida albicans in vaginal se-cretions from 2006 to 2010 was 16.7%(475/2840),including 87 cases combined Candida albicans, and the positive rate was 3.1%(87/2840). Conclusion The fungus convenient culture media is simple made, low cost, and well selected to Candida albicans, which can be used widely in grassroots medical institutions.