Autoantibody test results analysis of 225 patients with liver disease

SUN Ping; TANG Zhi-qin; MU Hong

doi:10.3969/j.issn.1674-7151.2014.02.009

Abstract：Objective To investigate the clinical value of autoantibodies detection in autoimmune liv-er disease(ALD) including autoimmune hepatitis(AIH), primary biliary cirrhosis(PBC) and primary scleros-ing cholangitis (PSC). Methods From January 2013 to February 2014, 225 patients from department of gas-troenterology, department of hepatobiliary surgery and transplant surgery were collected. Antinuclear antibody (ANA), antismooth muscle antibody(SMA) and anti-mitochondrial antibody(AMA) were detected by indirect immunofluorescence method. Liver kidney microsomal antibody(LKM), liver cytosol-1(LC-1), soluble anti-gen/liver pancreatic(SLA/LP) and AMA-M2 were tested by Europe and Mongolia blotting detection. The clin-ical datas were consulted and the detected results were analyzed statistically. Results The positive rates of ANA, SMA, AMA, LKM, AMA-M2, SLA/LP and LC-1 in patients with ALD were 77.78%, 7.41%, 55.56%, 7.41%, 48.15%, 7.41%and 3.70%, respectively. The positive rates of ANA, SMA, AMA, LKM, AMA-M2, SLA/LP and LC-1 in patients with other liver diseases were 26.26%, 4.55%, 11.11%, 0.51%, 5.05%, 2.53%and 1.52%, respectively. There were statistical significance in the differences of positive rates of ANA, AMA, LKM, AMA-M2 between ALD and other liver diseases group (Pall<0.05). Conclusion Au-toantibodies of ALD have a higher detection rate , and they provide a reliable basis for clinicians to identify vi-ral hepatitis, ALD and other liver diseases.