A Preliminary Study on the Effect of Lidocaine on Anabolism of Collagen Ⅱand Aggrecan in Cultured Chondrocytes

Yang Zhaohui

Abstract：Objective To investigate the effect of lidocaine on anabolism of cultured rabbit knee articular chondro-cytes.Methods Six anesthetized New Zealand big white rabbits were killed.We collected knee cartilage and separated articular chondrocytes,which were exposed to 2 mmoL/L lidocaine or saline for 3"6 or 9 days.The relative expression of CollagenⅡ and aggrecan of articular chondrocytes were assessed with RT-RNA.SPSS 13.0 software was used to an-alyze the data.Results RT-PCR assay showed the relative expression of CollagenⅡ mRNA exposed to 2 mmoL/L li-docaine for 3 " 6 or 9 days was (18.51±2.34)"(16.95±3.10)"(15.79±3.57),the ones exposed to saline for 3 " 6 or 9 days was (19.48±4.01)(18.76±1.96)(17.81±3.05)respevtively,there wasn’t statistically significant difference between lidocaine groups and saline groups (t=-0.32 "-0.45 "-0.72)(All P>0.05).The relative expression of aggrecan mRNA exposed to 2mmoL/L lidocaine for 3 " 6 or 9 days was(9.72±0.32)"(9.59±0.78)"(12.54±1.06), the ones exposed to saline for 3 " 6 or 9 days was (10.68±1.07)"(10.06±0.93)"(9.87±1.12)respevtively,there was statistically significant difference between lidocaine and saline groups at the ninth day (t=2.59,P<0.05).Conclu-sion The effect was limited on anabolism of cultured rabbit knee articular chondrocytes exposed to lidocaine at finite time and concentration.