Effect of Light Gene Regulation on Pathology Changes and Detrusor Receptors of Bladder after Spi-nal Cord Injury

Abstract：Objective To observe of the effect of light gene regulation on neurogenic bladder function recovery by the up-per-sacral spinal cord injury in rats. Methods 50 rats which had no abnormalities after urine flow dynamic test were random-ized and the 10 thoracic spinal cord complete transection animal model was set up,dividing into the control group,no blue light stimulation of spinal cord injury group and blue light stimulation of spinal cord injury group. The bladder morphological changes before and after the use of blue light stimulation were observed by HE staining technique. Furthermore,the express of M-AR and β-AR were tested by Real timePCR in the rat bladder detrusor. Results Compared with no blue light stimulation of spinal cord injury group,detrusor cells were relatively uniform shape and size while the more muscle fibers arranged in an orderly, closing to control group. Real timePCR technical results show that,compared with the control group,the expression of M2-AR mRNA and M3-AR mRNA in no blue light stimulation of spinal cord injury group was significantly higher(P ﹤ 0. 05)while the express was lower in the blue light stimulation of spinal cord injury group(P ﹤ 0. 05). β3-AR mRNA expression and M-AR expression were contrary. Conclusion Light gene regulation can repair the neurogenic bladder dysfunction of upper-sacral spi-nal cord injury by regulating the detrusor receptors.