Expression of Cancer ∕ testis Antigen﹣related Genes in Malignant Fibrous Histiocytoma

Abstract：Objective To detect the expression of cancer∕ testis antigen related genes in malignant fibrous histiocytoma and to investigate the possibility of applying the antigens as the target antigens for malignant fibrous histiocytoma specific im-munotherapy. Methods Tumor tissues and adjacent noncancerous tissues were collected in 27 patients diagnosed as malignant fibrous histiocytoma and received operation from April,2010 to January,2015. Among them,there were 17 male and 10 female with the average age(58. 7 ± 13. 5)years old(31 ~ 83 years old). According to staging,5 cases were staged in Ⅰ,7 in Ⅱ,11 in Ⅲ,4 in Ⅳ;14 cases were of pleomorphic,8 cases of giant cell and 5 cases of inflammatory out of 27 cases. Reverse-tran-scription polymerase chain reaction(RT-PCR)was adopted to investigate the expression of cancer-testis antigen related genes (WT1,MPP11,PRAME,RHAMM,NY-CO-38,G250,NY-ESO-1,HTERT,BAGE)of the tumor tissues and adjacent noncan-cerous tissues. Results The expression rates of cancer-testis antigen related genes in the 27 cases of malignant fibrous histio-cytoma were WT1 77. 8%(21 ∕ 27),MPP11 74. 1%(20 ∕ 27),PRAME 63. 0%(17 ∕ 27),RHAMM 59. 3%(16 ∕ 27),NY-CO-38 51. 9%(14 ∕ 27),G250 44. 4%(12 ∕ 27),NY-ESO-1 44. 4%(12 ∕ 27),HTERT 40. 7%(11 ∕ 27),BAGE 14. 8%(4 ∕27)respectively;almost none of the adjacent noncancerous tissues were positive for these genes. There was no significant corre-lation(P ﹥ 0. 05)between cancer-testis antigen related genes WT1 with the highest expression rate in malignant fibrous histio-cytoma and clinical correlation index(gender,age,histologic subtypes,clinical stages). Conclusion The cancer-testis antigen related genes(WT1 et al)which represent high expression in malignant fibrous histiocytoma are suitable and potential attack target for the active immunotherapy of malignant fibrous histiocytoma.