The Influence of Methylprednisolone on the Expression of Apoptosis Cytokine of Neuron after Acute Mechanical Injury
Abstract：Objective To research the influence of methylprednisolone on the expression of apoptosis cytokine in the cul-tured rat spinal neuron after actue mechanical injury. Methods Co-culture astrocytes and neurons were prepared in vitro. The astrocytes and neurons were co-cultured in the control group. And we used the scalpel to cut the neuron in order to make the damage model in the injury group:1uM methylprednisolone was mixed in culture solution in the mechanical damage model in the methylprednisolone group. The apoptosis related factors were tested. Compare expression of apoptosis related factor in 3 groups after cells were cultured 24 h. Results In injury control group,compared with sham control group,caspase-3,caspase-3 mRNA and Bax expression significantly increases(P<0. 05),while the ratio of the Bcl-2/Bax and the expression of Bcl-2 lower(P<0. 05). The expression of caspase-3,caspase-3 mRNA and Bax were less in methylprednisolone group compared with the injury control group(P<0. 05),while the Bcl-2 expression and the ratio of the Bcl-2/Bax elevated(P<0. 05). Conclu-sion Methylprednisolone can reduce the expression of apoptosis cytokine after neurons mechanical injury,and increase the rate of the Bcl-2/Bax,inhibit the apoptosis process of neurons.
Keywords：acute spinal cord injurymethylprednisoloneneuron culturemechanical damageapoptosis cytokine
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 516-520 )