The Expression of EGFR Protein,Gene Amplification and Its Clinical Significance in Osteosarcoma
Du Zhenguang
Xiao Mingming
Jing Shibing
Xu Shaonian
Abstract：Objective To investigate the expression of EGFR protein and gene amplification in osteosarcoma and evalua-tion of EGFR in osteosarcoma occurrence,development and prognosis. Methods PV-9000 two step method and in situ chemi-cal hybridization(FISH)were used to detect the expression of EGFR protein,gene amplification in 32 cases of osteosarcoma and 10 cases of osteochondroma in paraffin-embedded tissue chip material. Results The positive rate of EGFR expresion in osteosarcoma,osteochondroma were 84. 4% 、20. 0% . The positive rate of EGFR gene amplification were 75% and 20% . There were significant differences between osteosarcoma and osteochondroma in the expression and amplification of EGFR. The ex-pression and amplification of EGFR in osteosarcoma had no correlation with age ,gender ,or the location of the tumor( P >0. 05),but were correlated with metastasis and clinical stage(P < 0. 05). Conclusion EGFR is closely related to the occur-rence and development of osteosarcoma,and can be considered as an important index for determing the biological behavior of osteosarcoma.
Keywords：osteosarcomaEGFRimmunohistochemistryfluorescence
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 328-330,375 )