Exploratory study on the detection of hepatitis B virus nucleic acid by two fluorescent quantitative poly-merase chain reaction reagents
XU Xin
CHEN Min
ZHU Minzhi
Abstract：Objective:By comparing the test results of the two test reagents,the correlation between the results of quantitative detection of hepatitis B virus nucleic acid(HBV-DNA)by a domestic reagent(reagent A)and an impor-ted reagent(reagent B)was explored,so as to select a more suitable reagent for laboratory project development and clinical disease monitoring and treatment.Methods:ALL 95 cases of patients with hepatitis B clinically diagnosed in Changzhou Third Peoples Hospital from May 2024 to October 2024 were collected,and two reagents A and B were used for parallel detection,respectively.The detection results were compared and the correlation and difference between the two reagents were analyzed.Results:The detection rates of reagent A(90.53％)was higher than B reagent(82.11％),and there was no significant difference between the quantitative results(P=0.706).The quantitative results of reagent A was well correlated with B reagent,and the correlation co-efficient was 0.987,respectively,and differences were statistically significant(P＜0.001).Conclusion:Reagent A has good correlation with reagent B.There is no significant difference in the quantitative results.They can be used for quantitative detection of HBV-DNA in clinical laboratories to meet the antiviral requirements of hepatitis B patients.and basic requirements for treatment monitoring.
Keywords：hepatitis Bhepatitis B virushepatitis B virus DNA
Publication Date：2025-10-10
Online Publishing Date：2025-10-29(First online date of this platform, not the publication date of the document)
Pages：5( 770-774 )