Clinical study of intracytoplasmic sperm injection assisted by microlaser trepanning

WU Chengliang; CHEN Yaqiang; ZHANG Jie

Abstract：Objective:To observe the clinical study of intracytoplasmic sperm injection(ICSI)assisted by microlaser trepanning.Methods:A total of 202 patients treated with second-generation in vitro fertilization in reproductive center of Jiujiang Maternal and Child Health Hospital were enrolled from January 1,2022 to April 31,2024.According to dif-ferent treatment methods,patients were divided into routine ICSI group(hereinafter referred to as Group A,n=81,number of injected ovum:652),trepanningdepth＜50％group(hereinafter referred to as Group B,n=60,number of in-jected ovum:526)and trepanningdepth ≥50％group(hereinafter referred to as Group C,n=61,number of injected o-vum:535).Group A was treated with conventional ICSI,group B was treated with micro-laser drilling depth＜50％and group C was treated with micro-laser drilling depth ≥50％.After treatment,fertilization rate,(2PN)normal fer-tilization rate(2PN is after normal fertilization of the egg,the male and female prokaryotes appear),high-quality em-bryo rate,clinical pregnancy rateand blastocyst formation rate of three groups were compared.Results:The fertilization rate,(2PN)normal fertilization rate and high-quality embryo rate in group A were lower than those in group B and group C.The fertilization rate and high-quality embryo rate in groupB were lower than those in group C(P＜0.05),but there was no significant difference in(2PN)normal fertilization rate between the two groups(P＞0.05).The clini-cal pregnancy rate and blastocyst formation rate in group A were lower than those in group B and group C,and the clin-ical pregnancy rate and blastocyst formation rate in group B were lower than those in group C(P＜0.05).Conclusion:The application of ICSI assisted by microlaser trepanningin IVF can effectively improve fertilization rate,high-quality embryo rate,clinical pregnancy rate and blastocyst formation rate.