The correlation between salivary carbonic anhydrase Ⅵ and immune biomarkers and the severity of caries in young children with caries

LI Jing; WANG Fenying

Abstract：Objective:To analyze the correlation between carbonic anhydrase Ⅵ(CA-Ⅵ)and immune biomarkers in saliva and the severity of early childhood caries(ECC).Methods:One hundred and forty-six children with ECC(ECC group)were divided into severe early childhood caries(SECC)group(n=79)and non-SECC group(n=67)according to the severity of caries,and 100 healthy children were selected as the control group.The morning saliva sam-ples of the subjects were collected,and the levels of CA-Ⅵ,matrix metalloproteinase(MMP)-2,MMP-9,interleu-kin(IL)-6,tumor necrosis factor-α(TNF-α),secretory immunoglobulin A(sIg A),immunoglobulin(Ig)A,and IgG in saliva were detected.The correlation between they and the decayed missing and filled surface(DMFS),and the diagnostic value of SECC were analyzed.Results:The level of CA-Ⅵ in the saliva of the ECC group was lower than that of the control group(P＜0.05).The levels of MMP-2,MMP-9,1L-6,TNF-α,sIgA,IgG and IgA were higher than those in the control group(P＜0.05).The level of salivary CA-Ⅵ in the SECC group was lower than that of the non-SECC group(P＜0.05).The levels of MMP-2,MMP-9,IL-6,TNF-a,sIgA,IgA and IgG in the SECC group were higher than those in the non-SECC group(P＜0.05).Correlation analysis showed that saliva CA-Ⅵ was negatively correlated with DMFS in children with ECC(r=-0.516,P＜0.05).The levels of salivary MMP-2,MMP-9,IL-6,TNF-α,sIg A,IgA and IgG were positively correlated with DMFS(r=0.558,0.474,0.420,0.375,0.434,0.336,0.373,P＜0.05).Possion regression analysis showed that saliva CA-Ⅵ,MMP-2,MMP-9,IgA and IgG were the influencing factors of DMFS(P＜0.05).ROC curve analysis showed that the area under the curve(AUC)of identification SECC of CA-Ⅵ,MMP-2,MMP-9,1L-6,TNF-α,sIg A,IgA and IgG were0.757,0.835,0.788,0.745,0.660,0.740,0.747 and 0.696,respectively.Conclusion:The level of CA-Ⅵ in the saliva of children with ECC is significantly decreased,and the level of immune biomarkers is significantly increased,which is closely related to the severity of caries.