HPLC-DAD fingerprinting study on the herbs of different origins of Ziziphi jujuba kernel
CHENG Jie
ZHANG Chenwu
Abstract：Objective:To establish the high performance liquid chromatography-diode array detector(HPLC-DAD)fingerprinting method of the herbs of different sources and origins of sour jujube nut.Methods:The compounds in 12 batches of herbs of different origins were extracted by ultrasonic centrifugation,and the HPLC-DAD fingerprints were detected at 204 nm through gradient elution procedure,and the fingerprints were analyzed and evaluated.Results:Through the construction and analysis of the fingerprints,a total of 27 common peaks were identified,5 active com-pounds were designated,and the relative retention times in the fingerprints were close to each other with a high degree of similarity.Conclusion:The HPLC-DAD fingerprinting assay of sourjujube nut herb established in this paper is easy to process,fast to detect,convenient to operate,and can be used to identify,classify and evaluate unknown samples.
Keywords：sour jujube kernelfingerprintinghigh performance liquid chromatography-diode array detectoreval-uation of similarity of chromatographic fingerprints of Chinese medicine
Publication Date：2024-11-10
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 846-851 )