The expression of brain-derived neurotrophic factor proteins in the young mice brain after repeated sevoflurane anesthesia

XU Tiantian

Abstract：Objective:To evaluate the changes in the expression of neurotrophin protein in the brain of young mice with cognitive dysfunction induced by repeated inhalation of sevoflurane anesthesia.Methods:SPF healthy female C57BL/6J young mice at the birth of 6 days were divided into 2 groups using a random number table method:control group(C group)and multiple inhalations of sevoflurane anesthesia group(Sev group).Sev Group was given 3%sevoflurane+60%O2,2 h a day for 3 days.The control group was only given 60%O2 in the same modelling box,2 h a day interrupted for 3 days,and the experiment was carried out at the same time every day.At the end of modelling,the young rats were returned to the mother rat cage after breathing was stable and completely awake.After modelling,the young mice were fed for 30 days,and the long-term learning and memory function of the mice was detected.At the end of modelling,6 mice were sacrificed after deep anesthesia,and the hippocampus was obtained for determination of the expression of brain-derived neurotrophic factor-related proteins[cAMP reaction element(CREB),Neurotrophin 3(NT-3),Neurotrophin 4(NT-4)and tyrosine kinase B(Trkb)]and synaptic plasticity protein(PSD95)(by Western blot).Results:Compared with the C group,the escape latency of the young mice in the Sev group was significantly pro-longed(P＜0.05),and the number of platform crossings was reduced(P＜0.05).The results of enzyme-linked im-munosorbent assay(ELISA)showed the expression of inflammatory factors in serum was increased in the Sev group(P＜0.05).The expression of brain-derived neurotrophic factor proteins(CREB,BDNF,NT-3,NT-4 and Trkb)and synaptic plastic-related protein PSD95 in the hippocampus of young mice in the Sev group were decreased(P＜0.05).Conclusion:The cognitive impairment induced by repeated inhalation of sevoflurane anesthesia in young mice may be re-lated to the decreased expression of brain neurotrophic factor-related proteins(CREB,BDNF,NT-3,NT-4 and Trkb proteins)and hippocampal synaptic plasticity-related protein PSD95.