To investigate the effect of microsurgical ligation on sperm DNA fragmentation index in patients with varicocele based on oxidative stress effect

XIONG Yong; MA Ling; ZHENG Xiaoting; ZHANG Mingliang; XIONG Qi

Abstract：Objective:To investigate the effect of microsurgical ligation of spermatic vein on sperm DNA fragmenta-tion index(DFI)and clinical outcome in patients with varicocele(VC).Methods:A total of 80 patients with VC accom-panied by abnormal sperm DNA fragmentation and treated with assisted reproductive technology(ART)in the Depart-ment of Reproductive Andrology,Nanchang Reproductive Hospital were selected and randomly divided into two groups according to the random number table method.There were 40 cases in the observation group and 40 cases in the control group.The observation group was treated with microsurgical ligation of spermatic vein and combined with levocarnitine oral solution after operation.The control group was treated with levocarnitine oral solution alone.The treatment course of the two groups was 3 months.The changes of sperm DNA fragmentation index,sperm concentration,percentage of forward movement sperm(PR)and clinical outcome indexes were observed in the two groups.Results:After treat-ment,the total effective rate of the two groups was compared,and the observation group was higher than the control group(P＜0.05).Compared with the sperm treatment of the two groups,the DNA fragmentation index of the observa-tion group was significantly lower than that of the control group(P＜0.05),and the sperm concentration of the obser-vation group was significantly higher than that of the control group(P＜0.05).The percentage of PR sperm(forward motile sperm)was significantly higher than that of the control group(P＜0.01).In the comparison of clinical out-comes,the fertilization rate,cleavage rate,high-quality embryo rate,capsular success rate and pregnancy rate in the observation group were higher than those in the control group(P＜0.05).Conclusion:The combination of antioxidant therapy after varicocele ligation can significantly improve the sperm quality of patients with varicocele,repair the com-plete structure of sperm DNA,and promote the clinical outcome of in vitro fertilization(IVF).The curative effect is better than that of antioxidant therapy alone.It is worthy of clinical promotion and treatment for male infertility pa-tients with varicocele.