Effect and mechanism of liraglutide on podocyte apoptosis induced by high glucose

RONG Qingfeng; GUO Na; HAN Le

Abstract：Objective:To investigate the effect of glucagon like peptide-1 receptor(GLP-1R)agonists on podo-cyte apoptosis induced by high glucose(HG)and its possible mechanism.Methods:According to different glucose con-centrations and interventions,podocytes MPC5 cells were assigned to the following 7 groups:normal glucose group(NG),high glucose group(HG),mannitol group(MA),HG+silent information regulator 1(SIRT1)agonist group(HG+RSV),HG+SIRT1 agonist+SIRT1 inhibitor group(HG+RSV+S);HG+glucagon-like peptide-1 receptor(GLP-1R)agonist group(HG+LG),HG+GLP-1R agonist+GLP-1R inhibitor group(HG+LG+E).Podocyte apoptosis rate was assessed utilizing flow cytometry.The expression level of cytoskeletal protein synaptopodin was e-valuated through the utilization of confocal fluorescence imaging technique.The expression of GLP-1R and SIRT1 was assessed using Western blot analysis.Results:High glucose increased the podocytic apoptosis rate,decreased expres-sion of synaptopodin,SIRT1,and GLP-1R(all P＜0.01).SIRT1 agonist(RSV)attenuated the podocytic apoptosis rate and upregulated SIRT1 expression,And SIRT1 specific antagonists block the protective effect of SIRT1 agonists on podocytes.The podocytic apoptosis rate was reduced by the administration of GLP-1R agonist(LG),accompanied by increased expression of both SIRT1 and GLP-1R.However,GLP-1R specific antagonists blocked the protective effect of GLP-1R agonists on podocytes.Conclusion:GLP-1R agonists prevent podocytes injury induced by High glu-cose.The possible mechanisms may involve the GLP-1R/SIRT1 signaling pathway.