The mechanism of sodium cantharidinate in inducing apoptosis related gene expression in human cervi-cal cancer Hela cells

HUA Jinren; CHENG Huiming; HUANG Xuemei; CHEN Jin; TU Yunxia; WANG Liqun; PAN Mei

Abstract：Objective:To investigate the mechanism of sodium cantharidinate in inducing apoptosis related gene ex-pression in human cervical cancer Hela cells.Methods:Purchase one human cervical cancer Hela cell line from January 2023 to December 2023,culture it until the logarithmic growth phase,and start the experiment.Divide it into four groups,add sodium cantharidinate 0 and 4 separatelyμmol/L,8 μmol/L,16 μmol/L,set as blank control group and 4 μmol/L group,8μmol/L group,16μmol/L group,5 wells were set for each concentration(with a sample size of 5 in each group).Cell proliferation was detected using cell viability assay(CCK-8),cell apoptosis was detected using flow cytometry,and the expression of B lymphoblastoma 2(BCL-2),human apoptosis regulatory factor(Bax),and human cysteine protease 3(Caspase-3)was detected using real-time fluorescence quantitative polymerase chain reaction(Real time PCR).Results:The cell proliferation inhibition rate,apoptosis rate and relative expression level of Caspase-3 in 4 μmol/L Group,8 μmol/L Group and 16 μmol/L Group were higher than those in the blank control group,while the relative expression level of BCL-2/Bax was lower than that in the blank control group,the difference was signifi-cant(P＜0.05).With the increase of the concentration of sodium cantharidin,the proliferation inhibition rate,apoptosis rate and Caspase-3 of human cervical carcinoma Hela cells also increased,and the relative expression of BCL-2/Bax gradually decreased(P＜0.05).After treated with sodium cantharidin,the cells shrank,became round,and the edge of the cells sprouted to form many garland-like apoptotic bodies.Conclusion:Sodium cantharidinate has a dose-depend-ent effect on inhibiting the proliferation and promoting apoptosis of human cervical cancer Hela cells.Its regulatory effect on cell apoptosis may be related to the regulation of BCL-2,Bax,and Caspase-3 expression.