Rapid determination of 10 synthetic pigments in Chinese patent drug by anion exchange SPE-HPLC
REN Rongjun
MAO Yiping
TAO Yong
LU Daliang
Abstract：Objective: To establish a high performance liquid chromatography method for rapid determination of 10 kinds of artificial pigments including amaranth, carmine, allura red, erythrosine, brilliant crocein, new fuchsine, rhodamine B, sudanⅠ, scarlet 808, sudan Ⅳ in Chinese patent drug. Methods: The sample were ultrasonic extracted by 70％ methanol, then purified by anion exchange solid phase extraction (SPE). A reversed phase column Agilent-C18 (250 mm × 4. 6 mm, 5 μm) was used. Taking gradient elution of methanol-0. 02 mol/L ammonium acetate solution as the mobile phase with the application of diode array detector. The flow rate was 1. 0 m L/min, the wavelength for detection was 520 nm, and the column temperature was35 ℃. Results: Ten pigments presented in xinnaokang capsule possessed good linear correlation in the concentration range from10 μg/L to 100 μg/L, their quantitative detection limits ranged from 1. 05 μg/m L to 5. 55 μg/m L and their recoveries ranged from 83. 6％ to 99. 2％, the RSDs ranged from 1. 2％ to 3. 6％. Conclusion: This method is simple, accurate and reliable, with good reproducity, which can be used for rapid determination of multiple red pigments in Chinese patent drug.
Keywords：anion-exchange solid phase extractionhigh performance liquid chromatographychinese patent drugsynthetic pigments
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 127-131 )