Changes of nuclear factor - κB and intercellular adhesion molecule - 1 in rat liver exposed to inter-mittent hypoxia and the investigation on the effect of Tempol
JIANG Qiufang
Abstract：Objective:To study the effect of intermittent hypoxia on nuclear factor - κB(NF - κB)and intercellular adhe-sion molecule - 1(ICAM - 1)of rat liver and the investigation on the effect of Tempol. Methods:To establish a chronic inter-mittent hypoxia(CIH)animal model in rats,in order to mimic the intermittent hypoxia/ re - oxygenation(IH/ ROX)of obstruc-tive sleep apnea syndrome in humans. 32 healthy male Wistar rats were randomly assigned to 4 groups:conventional intermit-tent hypoxia group(CIH group),intermittent hypoxia Tempol treatment group(CIH + T group),intermittent hypoxia normal sa-line matched group(CIH + NS group),and normoxia control group(NC group),each group of eight rats. The frequency of every CIH group was 30 times/ hour,and the minimum oxygen concentration was 5% . After the experiment,we measured the levels of nuclear factor - κappaB(NF - κB)and ICAM - 1 in rat liver homogenate. Results:Compared with CIH group,CIH + NS group,the levels of NF - κB and ICAM - 1 in CIH + T group were decreased(P all < 0. 05),compared with NC group,the level of NF - κB in CIH + T group was higher than in NC group(P < 0. 05),but the level of ICAM - 1 in CIH + T group was not sig-nificant with in NC group(P > 0. 05). Conclusion:Chronic intermittent hypoxia can cause liver inflammatory injury in rats. Tempol could prevent the injury of inflammation of CIH to the rat liver through scavenging ROS by its anti - oxidative effect.
Keywords：intermittent hypoxiaobstructive sleep apnea syndromenuclear factor - κBTempol
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 835-838 )