Experimental study on insulin - like growth factor for early knee osteoarthritis of rats

GUO Tai; XUAN Wenxiao; LI Shuwei; BI Shuxiong

Abstract：Objective:To study the rabbit knee joint cavity injection of insulin - like growth factor 1(IGF - 1),the effect of early treatment of knee osteoarthritis. Methods:Twenty New Zealand white rabbits were randomly divided into 3 groups:group A(6)as the normal control group;Group B(7)for knee osteoarthritis treatment group;group C(7)for knee osteoarthritis control group. Among them,group B:working liquid injection of IGF - 1,1 mL each time,once a week,a total of injection of 4 weeks. Intra - articular injection of Group A and Group C:the same dose of normal saline,once A week. A total of injection of 4 weeks. Observe each group after 4 weeks of cartilage and synovial tissue pathological gross morphology,HE staining,and Wa-kitani score,to detect joint fluid of interleukin - 1(IL - 1)concentration. Results:Generally visible to the naked eye observa-tion,Group B compared with Group C,cartilage repair is better. Visible organization after HE staining under observation,carti-lage cells survive Group B than in Group C,cartilage matrix structure integrity,synovial hyperplasia of fibrous tissue of the low-er significantly less. ELISA method to detect the concentration of the IL - 1 in joint fluid and Wakitani score,according to the comparison between each group results with statistical significance(P < 0. 05). Conclusion:The articular cavity injection of IGF - 1 on early rabbit knee osteoarthritis has a better therapeutic effect.