The effects of LKB1 gene down - regulation induced by RNA in prostate carcinoma cell
LIU Chaoying
TANG Wei
JIANG Li
XIONG Jing
DENG Wei
Abstract：Objective:To investigate the effect of Liver kinase B1 / Serine - Threonine Kinase II(LKBl/ STKll)gene si-lencing on expression of cyclinD1,Mcm2 and PCNA of human prostate carcinoma PC - 3 cells as well as the relevant mecha-nism. Methods:The shRNA expression plasmid of LKB1(LKB1 - shRNA1)was constructed and transfected to PC - 3 cellsby liposome - mediated transfection,while using the cells untransfected as blank group and those transfected with Vector as nega-tivegroup,and the well - transfected cells were screened out using G418. The transcription levels of LKB1,cyclinD1,Mcm2 and PCNA mRNAs in the transfected cells were determined by RT - PCR,while the expression levels of LKB1,cyclinD1,Mcm2 and PCNA proteins by Western blot. Testing after transfection cell proliferation ability by CCK8. Results:Compared with those of blank and shNC groups,the relative levels of LKB1,cyclinD1,Mcm2 and PCNAmRNAs and proteins in LKB1 - shRNA1 cells increased significantly(P ﹤ 0. 01). Conclusion:Gene silencing of LKB1could up - regulate the expression of cyclinD1, Mcm2 and PCNA inprostate carcinoma cells;LKB1 gene might be a potential target for study on the molecular mechanism of-proliferation of prostatecarcinoma.
Keywords：prostate carcinomaLKB1 Proteinmacrophage migration inhibitory factormini chromosome maintenance protein 2proliferating cell nuclear antigen
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 531-535 )