Effectof sevofluranepostconditioningon the express ofmitofusion - 1in myocardial ischemia - reperfu-sion in rats with diabetes

GAO Tingting; HAN Chongfang; LUO Min; WANG Xiaopeng; YU Jing; WANG Xiang

Abstract：Objective:Toexplore the effects of sevoflurane postconditioning on the express of mitofusion - 1 in diabetes rats with ischemia reperfusion injury Methods:Sixty healthy adult male Sprague - Dawley rats,weighing 200 ~ 240 g,were randomly divided into 4 groups(n = 15 each ):normalsham operation group(S group),diabetic sham operation group(DS group),dia-beticischemia - reperfusion group(DI/ R group)and diabeticsevoflurane postconditioning group(DSev group). The model of diabetes mellitus was induced by intraperitoneal streptozotocin 65 mg/ kg. The model of myocardial ischemia/ reperfusion was produced by occlusion of the anterior descending branch of left coronary artery for 30minutes followed by 120 minutes reperfu-sion in rats. Threading but not ligation was induced in S group and DS group. The anterior descending branch of left coronary artery was occluded for 30 minutes andreperfused for 120 minutes in DI/ R group and DSev group. 2. 5% sevoflurane was in-haled for 5 minutes starts from 29 min of ischemia in DSev group. After 120 minutes of reperfusion,rats were sacrificed and cardiac tissues were obtained. Myocardial infarct size was measured by TTC staining. Cardiomyocyte apoptosis was determined by TUNEL. The expression of Mfn1 protein levels was determined by immunohistochemistry. Results:Compared with S group, the cardiomyocyte apoptosis index was increased,the express of Mfn1 protein was decreased(P ﹤ 0. 05),the myocardial infarct size has no significant difference in DS group(P ﹥ 0. 05). The myocardial infarct size and the myocardiomyocyte apoptosis in-dex was significant increasedthe expression of Mfn1 protein was obviously decrease,in DI/ R groupand DSev group(P ﹤ 0. 05). Compared with DS group,the myocardial infarct size and the myocardiomyocyte apoptosis index was significant increased,the express of Mfn1 protein was obviously decreased in DI/ R group and DSev group(P ﹤ 0. 05). Compared with DI/ R group,the myocardial infarct size,the myocardiomyocyte apoptosis index and the express of Mfn1 protein,has no significant difference with DSev group.(P ﹥ 0. 05). Conclusion:The express of Mfn1was decreased in DS group than in S group. Ischemia reperfu-sion injury further decrease the expression of Mfn1. Sevoflurane postconditioning has nosignificant effect on Mfn1 with diabetes rats which was going with ischemia/ reperfusion injury.