Effect of pentazocine on the alteration of serum inflammatory factors in lower limb ischemia reper-fusion injury induced by tourniquet

WANG Lili; ZHOU Xiaoqi; YUAN Dajiang

Abstract：Objective:Theaimofthisstudywastoexploretheeffectofpentazocineonthechangesofseruminflammatory factors tumor necrosis Factor-alpha( TNF-α),interleukin-6( IL-6 ),interleukin-10( IL-10 )in lower limbischemia reperfusion injury induced by tourniquet. Methods:Forty patients,undergoing elective unilateral leg surgery using tourniquet, were enrolled and randomly assigned to control group(group C,n=20)and pentazocine treatment group(group P,n=20). Pa-tients in group P pretreated with pentazocine(0. 5 mg/kg,in 100 mL of 0. 9% saline,intravenously infused in 30 min)after anesthesia,while only the same volume of saline was infused intravenously in group C at the same schedule. The operations were carried out under spinal anesthesia. Record the heart rate,thesaturation of pulse oxygenand mean arterial pressureat the time of before inflating tourniquet(T0),before deflating tourniquet(T1),1 minute(T2),30 minutes(T3)and 24 hours(T4) after tourniquet deflation. Peripheral venous blood samples were collected at the time of T0,T3 and T4. Serum concentration of TNF-α,IL-6 and IL-10 were measured by using enzyme-linked immunosorbent assay( ELISA). Results:There were no statistical difference detected in the general status and thehemodynamic changes between the group C and P(P>0. 05). The concentration of TNF-αhave no variation in two group at the three moments(P>0. 05). Compared with group C,the level of <br> IL-6 in group P were significantly decreased at the time of T3 and T4(P<0. 05),the level of IL-10 in group P was in-creased at the time of T3(P<0. 05),while there was no stastistical difference of IL-10 in two group at the time of T4(P>0. 05). Conclusion:These results indicated that pentazocine could suppress the inflammatory reactions in lower limbischemia reperfusion injury induced by tourniquet,which may beneficial for patient recovery.