Isolation and purification of fibrinogen from external use
HUANG Fan
LIANG Xiao-ming
YANG Du-cai
ZHANG Li-ling
LIU Yu-liang
KUANG Qing-fen
Abstract：Objective:A new method for preparation of human fibrinogen from external use was established. Methods:The process to cryprecipitate as raw material,using alumina adsorption,acid precipitation,ion exchange chromatography,low tem-perature ethanol precipitation method to extract human fibrinogen. Reference the method of Chinese pharmacopoeia,the detec-ting fibrinogen from external use of the main indicators of dissolution time,coagulation activity,purity,human coagulation factor XⅢ titer. Results:The products prepared in this study have the advantages of short dissolution time,strong solidification activi-ty,high purity,and the product quality is higher than the national standard,and better than the similar products in the market. Conclusion:This study established the preparation of human fibrinogen,and can effectively improve the comprehensive utiliza-tion of plasma,with a high economic value and social value.
Keywords：fibrinogencryoprecipitateion exchange chromatography
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 923-925 )