The antibody preparation of protein AAX14401 and its determination
HU Shao-hua
ZHAO Zhen-li
SUN Guang
CHANG Ji-wu
Abstract：Objective:AccordingtoAAX14401,whichistranslatedbyAY887902integrityreadingframe,preparationof antibody named anti-AY to AAX14401,and determinate the anti-AY. Methods:Amino acid translated by ORF of AY887902 showed significant deviations of GSTP1 at amino acid sequence 40 to 52 which we used as template,then according to the de-mand of preparation of antibodies,synthetic polypeptide and inject into mouse to make its polyclonal antibody:anti-AY. Re-sults:Enzyme-linked immunosorbent assay( ELISA)results reviewed that titer of the polyclonal is 1:35 000,which sug-gested that the polyclonal antibody was eligibility to detect AAX14401. Conclusion:The antibody of anti-AY prepared of pro-tein AAX14401 appropriate for the demand,and make a well foundation for detect the functional of AY887902.
Keywords：antibodypreparationmutantAAX14401bladderneoplasms
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 683-684,685 )