Effects of the down-regulated expression of Survivin gene by RNA interference on proliferation a-bility of cervical carcinoma cell Hela

LI Yuan-hong; LI Wei-ping

Abstract：Objective:The expression of survivin gene was regulated down by the technology of RNA interference in cervi-cal cancer to observe the changes of Hela cell proliferation ability. Methods:Transfected by liposome when the cells were grown to about 70. 0% coverage,the cells were divided into 3 groups,which were blank group(non—transfection group Hela cells), negative control group( empty plasmid transfection group)and the experimental group( survivin—siRNA( +)plasmid trans-fection group ). After interfering RNA specific for survivin gene of the cell Hela was transfected into Hela cells by the method of liposome induction,the morphological changes of cell growth were observed at different times,simultaneously the growth con-ditions of the cell growth were recorded respectively from 1d p. i. to 5 d p. i. ,then the growth curve were drew up accordingly. Recorded the number of living cells at 24 h,48 h,72 h by MTT assay and analyzed the conditions of cell growth. Results:By comparising the experimental group where the growth rate slows down by RNA Interference and the blank group,the number of the blank group cells was significantly higher than of the experimental group 3 d p. i. to 5 d p. i.(3 d p. i. P<0. 05;4,5 d p. i. P<0. 01). MTT assay showed that there was no obvious difference between the the number of living cells of the experimental group and of the control group,negative control group 24 h p. i.(P>0. 05),however,viable count of the experimental group was significantly lower than of the control group and negative control group at 48h p. i and 72h p. i.(P<0. 01). Conclusion:survivin gene silencing could inhibit the malignant proliferation ability of cervical carcinoma cell Hela in vitro,meanwhile,favor cell apoptosis. The survivin may serve as the target for cervical cancer by gene therapy.