Study on improvement effect of hydrogen free radical water on depression-like behavior induced by chronic unpredictable mild stress
LI Xiaole
HAN Shifan
CHENG Junxiang
ZHU Ruifang
PAN Mengyi
YOU Kaiqi
WU Jing
ZHANG Hong
ZHANG Xiaohui
ZHANG Qian
ZHAO Yu
SONG Jie
FENG Yaoqing
DONG Wenjing
LIU Qia
Abstract：Objective:To observe the safety of hydrogen free radical water and its improvement effect on depression-like behavior in rats.Methods:20 Sprague Dawley(SD)rats of 6 weeks of age in which half was male and half was female were randomly divided into the experimental group and the control group based on body weight.During the experiment,high concentration hydrogen free radical water and equal amount of distilled water were given gavage,respectively.After gavage,the general conditions and body weight changes of the rats in each group were observed and recorded,and related pathological examinations were carried out.32 male SD rats of 4 weeks of age were randomly divided into the normal control group,model group,low concentration group,high concentration group based on body weight.Chronic unpredictable mild stress intervention was carried out for 6 weeks in the model group,low concentration group,high concentration group.Chronic unpredictable mild stress rat depression model was constructed.The effects of hydrogen free radical water on depressed rats was evaluated by observing the behavioral indexes and serum corticosterone.The short-term safety of hydrogen free radical water was preliminarily evaluated by comparing the results of blood routine,blood biochemistry and pathological sections of each group.Results:When the rats were of the same sex,there were no significant differences in the growth rate of body weight at the same time point and organ index of vital organs between experimental group and control group(P>0.05).After 6-week CUMS,rats showed depression-like behavior,which could be effectively alleviated in low concentration group and high concentration group.The blood routine results of each group were within the normal range.Although the level of alkaline phosphatase in low concentration group and high concentration group was lower than that in normal control group(P<0.05),there was no significant difference compared with model group(P>0.05).There were no significant differences in the comparison of γ-glutamyltransferase,urea,creatinine,aspartate aminotransferase,and serum albumin among normal control group,model group,low concentration group,high concentration group(all P>0.05).Microscopically,it was found that the degeneration and necrosis of renal tubular epithelial cells in the model group was more serious than that in the high concentration group.Minor inflammatory cell infiltration was observed locally in the submucosa of the bladder in the model group,but not in the high concentration group.No significant abnormalities were observed in the other organ tissues in the high concentration group compared with the normal control group.Conclusion:Under the experimental conditions,the results of this study don't indicate any acute or short-term toxic effects or toxicological profiles of hydrogen free radical water,and suggest that it may have a protective effect against chronic unpredictable mild stress-induced depressive-like behaviors,possibly with a dose-dependent effect.
Keywords：hydrogen free radical waterchronic unpredictable mild stressCUMSdepression-like behaviorsecuritycorticosteroneanimal experimentation
Publication Date：2024-11-25
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 4013-4018 )